FIGURE 5.

miR-149-5p accelerates goat hair follicle stem cell proliferation. (A) Representative images of the EdU assay of hair follicle stem cells at 24 h after transfection with NC, miR-149-5p mimics, anti-NC, and miR-149-5p inhibitors in GM. Bars, 100 μm. (B,C) Quantification of EdU-positive cells (n = 6). The ratio of EdU-positive cells was calculated as (EdU-positive cells/Hoechst stained cells) × 100%. (D–G) Hair follicle stem cells were transfected with NC, miR-149-5p mimics, anti-NC, and miR-149-5p inhibitors in GM, and cell cycle phases were analyzed at 48 h after transfection by flow cytometry (D,E) and counted (F,G). The results from each group are shown as the mean ± SEM of three independent replicates. Independent-samples t-tests were used for statistical analysis. Asterisks indicate significant differences. No asterisk means P > 0.05, *P < 0.05, and **P < 0.01.