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. 2020 Nov 11;11:526349. doi: 10.3389/fphar.2020.526349

Figure 5.

Figure 5

The representative western blot images of LC3β, HO-1, Beclin-1, p-AMPK, p62, p-mTOR and immunohistochemical image of LC3β showed that LDOP-1 induced autophagy via AMPK/mTOR signaling way in vivo. (A) The expression of LC3β, HO-1, Beclin-1, p-AMPK, p62, p-mTOR detected by Western blot. (B-G) Statistical analysis on LC3β, HO-1, Beclin-1, p-AMPK, p62, p-mTOR. Immunohistochemical image of LC3β (H) measured by immunohistochemical analysis (400×, brown yellow granules indicate positive reaction). Data are expressed as the mean  ±  SD of three independent experiments. #P < 0.05, ##P < 0.01, ###P < 0.001 compare the control group; *P < 0.05 and **P < 0.01 compare model group. LDOP-L stood for LDOP-1-L, LDOP-H stood for LDOP-1-H.