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. 2020 Nov 11;11:526349. doi: 10.3389/fphar.2020.526349

Figure 7.

Figure 7

Effects of ethanol and LDOP-1 on GES-1 cells viability measured by MTT assay, LDH release and mitochondrial membrane potential. Effects of ethanol (A) and LDOP-1 (B) on GES-1 cells were measured by MTT assay. LDH release (C) was measured by LDH Cytotoxicity Assay Kit. Mitochondrial membrane potential was detected by JC-1 assay Kit (D). Immunofluorescence image of JC-1 measured by immunofluorescence technique (E). In the mitochondrial membrane potential is high, JC - 1 gathered in the mitochondrial matrix (matrix), the formation of polymer (J - aggregates), can produce red fluorescence; When the mitochondrial membrane potential was low, jc-1 could not accumulate in the matrix of the mitochondria. At this time, jc-1 was monomer and could produce green fluorescence. Data are expressed as the mean ± SD of three independent experiments. #P < 0.05, ##P < 0.01, ###P<0.001 compare the control group; **P < 0.01, ***P < 0.001 compare model group, $$$P < 0.001 compared with compound C group. LDOP stood for LDOP-1.