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. 2020 Nov 10;61(13):16. doi: 10.1167/iovs.61.13.16

Figure 1.

Figure 1.

DEX or Veh temporally and differentially modulated specific integrin subunits and integrin adhesomes in hTM cells. Primary hTM cells were cultured on tissue culture plastics and treated with vehicle control (Veh) or 100 nM dexamethasone (DEX) in 1% FBS for 1, 3, 5, and 7 day(s). Protein was extracted for Western blot analysis. Veh and DEX were, respectively, normalized to baseline protein levels (time point 0 day). β-Actin was used as a housekeeping protein. Respective representative blot (top) and densitometric analysis (bottom) of (A) αV integrin, (B) β1 integrin, (C) β3 integrin, (D) β5 integrin, (E) integrin linked kinase, (F) focal adhesion kinase, and (G) phospho-focal adhesion kinase. Columns and error bars; means and standard error of mean (SEM). Two-way ANOVA with the Holm Sidak pairwise comparisons post hoc test was used for statistical analysis (n = 5 biological replicates). *P < 0.05, **P < 0.01, ***P < 0.001 for DEX versus Veh, given significant treatment and time interaction. ‡P<0.05 for DEX versus Veh, given significant main effect of treatment. #P < 0.05, ##P < 0.01, ###P < 0.001 for Veh versus Baseline protein. hTM, human trabecular meshwork.