Figure 2.

DEX or Veh temporally and differentially modulated Caveolin1 and Cavin1, major components of caveolae. Primary hTM cells cultured on tissue culture plastics were treated with vehicle control (Veh) or 100 nM dexamethasone (DEX) in 1% FBS for 1, 3, 5, and 7 day(s). Protein was extracted for Western blot analysis. Veh and DEX were each normalized to baseline protein levels (time point 0 day). β-Actin was used as an internal control. Respective representative blot (top) and densitometric analysis (bottom) of (A) Caveolin1 and (B) Cavin1. Columns and error bars; means and standard error of mean (SEM). Two-way ANOVA with the Holm Sidak pairwise comparisons post hoc test was used for statistical analysis (n = 5 biological replicates). ^‡‡P < 0.01 for DEX versus Veh, given significant main effect of treatment. ^†P < 0.05, ^†††P < 0.001 for DEX versus baseline proteins given significant main effect of treatment. ##P < 0.01 for Veh versus baseline protein. hTM, human trabecular meshwork.