Figure 3.

DEX or Veh temporally and differentially modulated key cytoskeletal proteins in hTM cells. Primary hTM cells were cultured on tissue culture plastics and treated with vehicle control (Veh) or 100 nM dexamethasone (DEX) in 1% FBS for 1, 3, 5, and 7 day(s). Protein was extracted for Western blot analysis. Veh and DEX were respectively normalized to baseline protein levels (time point 0 day). β-Actin was used as a housekeeping protein. Respective representative blot (top) and densitometric analysis (bottom) of (A) α-smooth muscle actin, (B) RhoA, (C) Rac 1/2/3, (D) Cdc 42, (E) vimentin. Columns and error bars; means and standard error of mean (SEM). Two-way ANOVA with the Holm Sidak pairwise comparisons post hoc test was used for statistical analysis (n = 5 biological replicates). *P < 0.05, **P < 0.01, ***P < 0.001 for DEX versus Veh, given significant treatment and time interaction. ^§§§P < 0.001 for DEX versus baseline protein given significant treatment and time interaction. ^†††P < 0.001 for DEX versus baseline protein given significant main effect of treatment. ##P < 0.01, ###P < 0.001 for Veh versus baseline proteins. hTM, human trabecular meshwork.