Table.
Antibodies for Western Blotting Analyses
| Antibody | Catalog Number | Stock Concentration, µg/mL | Dilution | Final Concentration, µg/mL | Source |
|---|---|---|---|---|---|
| FKBP5 | 12210 | 918.0 | 1/5000 | 0.184 | Cell Signaling Technology, Danvers, MA, USA |
| αV Integrin | 4711 | 53.0 | 1/2000 | 0.027 | |
| β1 Integrin (D2E5) | 9699 | 100.0 | 1/5000 | 0.020 | |
| β3 integrin (D7X3P) XP | 13166 | 66.0 | 1/5000 | 0.013 | |
| β5 integrin (D24A5) | 3629 | 338.0 | 1/5000 | 0.068 | |
| Focal adhesion kinase | 3285 | 31.0 | 1/2000 | 0.016 | |
| Phospho-focal adhesion kinase | 8556 | 16.0 | 1/2000 | 0.008 | |
| Integrin linked kinase | 3856 | 458.0 | 1/5000 | 0.092 | |
| Caveolin1 | 3267 | 689.0 | 1/5000 | 0.138 | |
| Cavin1 | 46379 | 30.0 | 1/5000 | 0.006 | |
| Cdc42 | 2462 | 585.0 | 1/5000 | 0.117 | |
| Rac1/2/3 | 2465 | 39.0 | 1/5000 | 0.008 | |
| RhoA | 2117 | 107.0 | 1/5000 | 0.021 | |
| Vimentin | 5741 | 45.0 | 1/5000 | 0.009 | |
| β-Actin | 4970 | 61.0 | 1/10000 | 0.006 | |
| α-SMA | A5228 | 2000.0 | 1/5000 | 0.400 | Sigma-Aldrich, St. Louis, MO, USA |
| Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, HRP | G-21234 | 1000.0 | 1/10000 | 0.100 | Invitrogen, Carlsbad, CA, USA |
| Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, HRP | G-21040 | 1000.0 | 1/10000 | 0.100 |