Table 1.

Blood volumes required for analytical procedures during the HIPS study

Blood sample analytics	Sampled blood volume, mL*
Screening and baseline samples
FVIII activity in plasma	1.8
Transcriptome profiling in PBMCs after short-term in vitro stimulation with FVIII; FVIII-specific antibody analytics	4.0
Epigenetic immune cell counting; whole-blood transcriptome profiling	2.0
Post-FVIII exposure samples
FVIII inhibitor testing; whole-genome profiling (once); FVIII mutation analysis (once)	2.7
Transcriptome profiling in PBMCs after short-term in vitro stimulation with FVIII; FVIII-specific antibody analytics	4.0
Epigenetic immune cell counting; whole-blood transcriptome profiling	2.0

PBMC, peripheral blood mononuclear cell.

^*The total blood volume sampled did not exceed 8 mL/kg body weight at any month during the study