Table 1.
Gene transfer activity of CD3-LVs on various cell types
| Vector | % Positive cells, mean ± SD | ||
|---|---|---|---|
| HUVEC* | HepG2* | Nalm-6† | |
| — | ND | ND | 0.02 ± 0.01 |
| TR66-LV | 0.29 ± 0.20 | 0.08 ± 0.11 | 0.05 ± 0.02 |
| TR66.opt-LV | 0.03 ± 0.04 | 0.09 ± 0.06 | 0.05 ± 0.03 |
| HuM291-LV | 0.23 ± 0.08 | 0.09 ± 0.13 | — |
| VSV-LV | 86.74 ± 3.73 | 32.53 ± 4.89 | 97.50 ± 0.37 |
ND, not detectable.
*
HUVEC and HepG2 cells (n = 2 technical replicates per condition) were transduced with 0.8 to 1 × 1010 CD3-LV particles or 9 × 106 VSV-LV particles and analyzed for GFP expression 4 days after vector incubation.
†
Nalm-6 cells (n = 3 different vector stocks per condition) were transduced with 5 to 8 × 109 CD3-LV particles or 4 × 108 VSV-LV particles and analyzed for CAR expression 6 days after vector incubation.