Skip to main content
. 2020 Nov 24;4(22):5810–5824. doi: 10.1182/bloodadvances.2020003299

Figure 3.

Figure 3.

Embryonic PAR2 regulates fetal liver macrophages. (A) Fetal livers were collected from PAR2+/− and PAR2−/− embryos at E15.5, resulting from the mating of a PAR2+/− mother with a PAR2−/− father. Each point in the graph is the average F4/80 area from ≥8 field of views per mouse. Scale bar, 50 µm. Fetal livers were collected at E13.5 (B) and at E15.5 (C) from different timed mating strategies of PAR2 signaling–deficient mice, and mRNA gene expression for macrophage markers and regulators of apoptotic cell clearance capacity was analyzed. (D) Fetal livers were collected at E15.5 from mating of heterozygous FVII-deficient mice (WT/F7KO), and mRNA expression of FVII and macrophage differentiation markers was determined for WT (WT/WT) and FVII-deficient (F7KO/F7KO) embryos. All data are mean ± standard deviation. *P < .05, **P < .01, ***P < .001, ****P < .0001, Mann-Whitney U test for Nr1h3 (B) and Id3 (D); all other P values were calculated using the Student t test.