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. 2020 Nov 20;13:4495–4505. doi: 10.2147/DMSO.S278267

Figure 3.

Figure 3

Effects of RSV on SphK1/S1P2 pathway in LPS-induced RMCs’ proliferation. RMCs were treated with the same method as above. (A) RMCs were stimulated by LPS (100 ng/mL) for different periods of time, from 0~48 h, and then the expression of SphK1 and S1P2 was measured by Western blot. (B) RMCs were stimulated with LPS for 24 h with or without RSV at 20 μM, SK-II or JTE-013. Then the expression of SphK1 and S1P2 were examined. (C and D) SphK1 activity and S1P content were measured in LPS-induced RMCs for 24 h with or without different concentrations of RSV, SK-II or JTE-013 by SphK activity assay kit and ELISA kit, respectively. (E) RMCs were stimulated with LPS for 24 h with or without RSV at 20 μM, SK-II or JTE-013. Then FN and ICAM-1 expression were examined. (F) RMCs were stimulated with LPS for 12 h with or without RSV at 20 μM, SK-II or JTE-013. Then iNOS expression was examined. Experiments were performed in triplicate with similar results. Data are means ± SEM. #P<0.05 vs Control, ##P<0.01 vs Control; *P<0.05 vs LPS-treated group, **P <0.01 vs LPS-treated group.