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. 2020 Oct 13;24(21):12341–12354. doi: 10.1111/jcmm.15720

FIGURE 3.

FIGURE 3

MEG3 decrease miR‐233 expression to up‐regulate TRAF6. A, RT‐qPCR detection of the expression of miR‐223 in myocardial tissues and infiltrated macrophages of viral myocarditis (VMC) mice. B, RT‐qPCR detection of the expression of miR‐223 in MEG3‐silenced VMC mice. C, Binding site between miR‐223 and TRAF6 predicted by Targetscan. D, Targeting relationship between miR‐223 and TRAF6 detected by dual‐luciferase reporter assay. *P < .05 vs NC mimic. E, TRAF6 expression after alteration of miR‐233 level measured by RT‐qPCR. F, TRAF6 expression after alteration of miR‐233 level monitored by Western blot analysis. *P < 0.05 vs treatment with NC‐inhibitor; # P < 0.05 vs treatment with NC‐mimic. G, miR‐223 expression after alteration of MEG3 level examined by RT‐qPCR. H, TRAF6 expression after alteration of MEG3 level measured by RT‐qPCR. I, TRAF6 expression after alteration of MEG3 level monitored by Western blot analysis. *P < 0.05 vs treatment with si‐NC; # P < 0.05 vs treatment with oe‐NC. The measurement data were described as mean ± SD. Comparisons between two groups were made by unpaired t test. The experiment was performed at least three times. The control group received no treatment