FIGURE 5.

MEG3 down‐regulation suppresses the NF‐κB pathway via miR‐223 and TRAF6. A, NF‐κB DNA binding activity after MEG3 was down‐regulated. B, Expression of the NF‐κB pathway components after down‐regulation of MEG3 determined by Western blot analysis. *P < 0.05 vs control mice; ^# P < 0.05 vs viral myocarditis (VMC) mice injected with sh‐NC. C, NF‐κB DNA binding activity after miR‐223 over‐expression and TRAF6 down‐regulation. D, Expression of the NF‐κB pathway components after miR‐223 over‐expression or TRAF6 knockdown determined by western blot analysis. *P < 0.05 vs VMC mice injected with NC agomir; ^# P < 0.05 vs VMC mice injected with sh‐NC. All data were presented as measurement data and expressed as mean ± SD. Unpaired t test was made in comparisons. N = 5 or 10