TABLE 1.

Primer sequences of RT‐qPCR

Genes	Primer sequence
MEG3	F: 5′‐GGGAGCAGCTATGGATCACC‐3′
	R: 5′‐ATAGCGCCCCCTATTCATGC‐3′
miR‐223	F: 5′‐GCGTGTATTTGACAAGCTGAGTT‐3′
	R: 5′‐GTGTCAGTTTGTCAAATACCCCA‐3′
TRAF6	F: 5′‐ATTCTCGACCAGTCTGAAG‐3′
	R: 5′‐ATGAAGGTTCCCTGTCT‐3′
iNOS	F: 5′‐CCCTTCAATGGTTGGTACATGG‐3′
	R: 5′‐ACATTGATCTCCGTGACAGCC‐3′
TNF‐α	F: 5′‐TCTTCTCATTCCTGCTTGTGG‐3′
	R: 5′‐GGTCTGGGCCATAGAACTGA‐3′
Arginase‐1	F: 5′‐AGACAGCAGAGGAGGTGAAGAGTAC‐3′
	R: 5′‐GGTAGTCAGTCCCTGGCTTATGGT‐3′
Fizz‐1	F: 5′‐CGTGGAGAATAAGGTCAAGGAAC‐3′
	R: 5′‐AGCACACCCAGTAGCAGTCATC‐3′
NLRP3	F: 5′‐AGATGCTGGAATTAGACAACTG‐3′
	R: 5′‐CATTTCACCCAACTGTAGGC‐3′
ASC	F: 5′‐GAAGCTGCTGACAGTGCAAC‐3′
	R: 5′‐TGTGAGCTCCAAGCCATACG‐3′
CAPS1	F: 5′‐ACCACTCGTACACGTCTTGC‐3′
	R: 5′‐TGGGCAGGCAGCAAATTCTT‐3′
U6	F: 5′‐GCTTCGGCAGCACATATACTAAAAT‐3′
	R: 5′‐CGCTTCACGAATTTGCGTGTCAT‐3′
β‐actin	F: 5′‐CATCCGTAAAGACCTCTATGCCAAC‐3′
	R: 5′‐ATGGAGCCACCGATCCACA‐3′

Abbreviations: ASC, apoptosis‐associated speck‐like protein containing a CRAD; iNOS, myo‐inositol‐1‐phosphate synthase; MEG3, maternally expressed 3; miR‐223, microRNA‐223; NLRP3, NLR family pyrin domain‐containing 3; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction; TNF‐α, tumour necrosis factor‐alpha; TRAF6, TNF receptor‐associated factor 6.