FIGURE 2.

TSP‐2 induces cell migratory potential by MMP9 induction in human osteosarcoma cells. A, B, Osteosarcoma cells (MG63 and HOS) were incubated with TSP‐2 (30 ng/mL) for 24 hours, and the qPCR analysis was conducted to determined levels of MMP‐1, MMP‐2, MMP‐3, MMP‐7, MMP‐9, MMP‐12 and MMP‐13 mRNA expression. C, D, Osteosarcoma cells were incubated with different concentration of TSP‐2 (0‐30 ng/mL) for 24 hours and subsequently examined MMP‐9 expression levels by qPCR and Western blot assay. E, F, Osteosarcoma cells were incubated with TSP‐2 (30 ng/mL) for different time courses (0, 6, 12 and 24 hours), followed by investigation of MMP‐9 expression level through qPCR and Western blot assay. All bars represent the mean ± SD. The asterisks indicate that the data are significantly different from the control without TSP‐2 treatment. *Represents P < .05 compared with the respective control using one‐way analysis of variance (ANOVA) followed by Bonferroni's post hoc test