FIGURE 3.

Corticosteroids suppressed NLRP3‐inflammasome activation via inhibiting NF‐κB pathway. Peritoneal macrophages from WT mice were treated with various dosages of dexamethasone before challenged with LPS (500 ng/mL) for 4 h and then nigericin (Nig) (20 µmol/L) for 15 min. A, Western blot analysis showed that dexamethasone inhibited caspase‐1, IL‐1β in culture supernatant. The expression of NLRP3‐inflammasome, pro‐caspase‐1, pro‐IL‐1β and NF‐ĸB (p65) also suppressed by dexamethasone, which were in dose‐dependent manner. B, Dexamethasone exerted a protective effect on LPS‐induced cell damage. C, Transcription of TNF‐a was inhibited by various dosages of dexamethasone. D, Transcription of Caspase‐1 was inhibited by various dosages of dexamethasone. E, Transcription of IL‐1β was inhibited by various dosages of dexamethasone