FIGURE 1.

A, Identification of the transplanted hADSCs and their cardiac phenotype from the border zone 3 d after infarction. The grafted cells were identified, and their human nature was established by positive immunostaining with anti‐human mitochondrial antibodies (brown). Human stem cells were observed at implanted area of transplantation groups but not the vehicle. More grafted cells were identified in the infarcted group pre‐treated with BP compared with hADSCs alone. A, young; B, young/ADSC; C, old; D, old/ADSC; E, old(BP)/ADSC. B, Engraftment of hADSCs. qPCR for human Alu was performed. Alu‐DNA index (amount of Alu‐amplified DNA in hADSC recipient rats relative to that detected in young infarcted rats). The number of animals in each group is indicated in parentheses. *P < .05 compared with young/ADSC; ^† P < .05 compared with the infarcted group treated with old/ADSC