FIGURE 2.

SLC52A3 rs13042395 C > T change down‐regulated its mRNA expression via changing binding affinity with MEIS1. (A) In normal gastric tissue, samples with rs13042395 CT genotype had significantly higher expression level of SLC52A3 compared to CC genotype based on GTEx database. (B‐C) In GCa tissues and normal gastric tissues, the expression of SLC52A3 was negatively significantly associated with the MEIS1 gene from GEPIA online tools based on TCGA database. (D‐F) Knock‐down MEIS1 in BGC823 cells using three different siRNA led to the decreased expression of SLC52A3 mRNA and protein. GCa, gastric carcinoma; GTEx, The Genotype‐Tissue Expression project; TCGA, The Cancer Genome Atlas