Figure 2.

Effects of lincFOXF1 on osteosarcoma cell proliferation, migration and invasion in vitro and in vivo. A, lincFOXF1 expression in MNNG‐HOS and 143B cells transfected with pcDNA‐lincFOXF1 or lincFOXF1‐specific siRNA was detected by qRT‐PCR. B, CCK8 assays were used to determine the viability of transfected osteosarcoma cells. C, Colony‐formation assays were performed to assess the colony‐formation ability of si‐lincFOXF1‐ or pcDNA‐lincFOXF1‐transfected osteosarcoma cells. D, Wound‐healing assays were employed to investigate the migratory capacity of si‐lincFOXF1 or pcDNA‐lincFOXF1‐transfected osteosarcoma cells. (E) and (F) Transwell assays showed that lincFOXF1 knockdown promotes osteosarcoma cell migration and invasion and that lincFOXF1 overexpression inhibits osteosarcoma cell migration and invasion. G, lincFOXF1 inhibits osteosarcoma cell metastasis in vivo. All experiments were performed in triplicate. Bars: SD; ^* P < 0.05 and ^** P < 0.01