Figure 3.

lincFOXF1 physically interacts with PRC2 components EZH2, and lincFOXF1‐induced inhibition of migration and invasion in osteosarcoma cells requires EZH2. A, RNA expression levels of nuclear and cytosolic fractions were measured by qRT‐PCR. GAPDH was used as a cytosolic marker, and U1 was used as a nuclear marker. B, RIP assays showed that lincFOXF1 binds to the EZH2 complex in MNNG‐HOS and 143B cells. C, EZH2 expression in MNNG‐HOS and 143B cells after transfection with si‐EZH2 was detected by qRT‐PCR. D, The effect of pcDNA‐lincFOXF1 expression and co‐treatment with si‐EZH2 on the migration of MNNG‐HOS and 143B cells was investigated by transwell assays, and overexpression of lincFOXF1 could not reduce the migration of osteosarcoma cells in the presence of EZH2 function blockade. All experiments were performed in triplicate. Bars: SD; ^* P < 0.05 and ^** P < 0.01