Figure 4.

GIT1 is a potential target of lincFOXF1 and inversely correlated with lincFOXF1 expression in osteosarcoma tissues. (A) and (B) qRT‐PCR was used to detect expression of cell adhesion and extracellular matrix molecules, including N‐cadherin, Intergrin, CD44, ICAM‐1, Vimentin, Fibronectin, GIT1, and host gene FOXF1 after lincFOXF1 was knocked down or overexpressed in MNNG‐HOS cells. C, Western blot analyses were performed to confirm the gene expression in lincFOXF1‐overexpressing 143B cells or knocked down MNNG‐HOS cells compared with control cells. D, The inverse correlation between lincFOXF1 expression (ΔCt value) and GIT1 expression (ΔCt value) was analysed by qRT‐PCR in osteosarcoma tissues. All experiments were performed in triplicate. Bars: SD; ^* P < 0.05 and ^** P < 0.01