Figure 6.

SP promotes the coverage of vasculature by transplanted MSCs via PDGF‐PDGFR signalling. (A) AG1295 was added to MSCs with SP to inhibit PDGF‐PDGFR signalling. Immunofluorescence staining of skin tissues with anti‐human α‐SMA and anti‐mouse CD31 to detect localization of transplanted MSCs in the host vasculature. (B) Transplanted human α‐SMA (+) MSCs encircling vessels of total mouse CD31 (+) vessels were quantified. (C) Histological analysis of the vasculature was performed via staining for CD31 (D) Quantification of CD31 (+) vasculature was performed at 7 post‐transplantation. (E) Immunofluorescence staining of skin tissues with anti‐human α‐SMA and anti‐mouse CD31 in AG1295‐treated mice. Values of P < .05 were considered statistically significant (*P < .05). The data are represented as mean ± SD of three independent experiments. N = 8 for each group. Scale bar100 μm. Yellow arrow head: Transplanted human α‐SMA (+) MSCs‐ encircled vessels. White arrow head: vessel without transplanted human α‐SMA (+) MSCs