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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Dev Biol. 2019 Oct 3;457(1):13–19. doi: 10.1016/j.ydbio.2019.10.001

Figure 4: Conditional β4 depletion results in impaired alveologenesis and milk production in vivo.

Figure 4:

A) Immunostaining of Cre Recombinase in WAP-Cre+ glands from mice at day 2 of lactation. B) Immunostaining of ITGB4 and ITGA6 WAP-Cre+ and WAP-Cre− glands from mice at day 2 of lactation. (Scale bar = 20μm) C) H&E staining of WAP-Cre+ and WAP-Cre− glands from mice at day 17 of pregnancy and day 2 of lactation (Scale bar = 500μm). D) Whole-mount, H&E, and cleaved caspase 3 staining of WAP-Cre+ and WAP-Cre− glands from mice at day 2 of lactation. (Scale bar = 100μm) E) β-casein protein expression was analyzed using immunostaing and mRNA expression was quantified by qPCR in WAP-Cre+ and WAP-Cre− glands at day 2 of lactation. (Scale bar = 20μm) F) Npt2b expression was quantified by qPCR in WAP-Cre+ and WAP-Cre− glands at day 2 of lactation. G) Milk uptake in pups born to WAP-Cre+ and WAP-Cre− mice was compared at 2 days post birth.