a. Confocal images of single pC1 neurons in the female brain, as in Fig. 3d, marked with arrowheads to indicate the presence (solid) or absence (open) of subtype-specific branches. b, Confocal images of neurobiotin-filled pC1 neurons from which whole-cell patch recordings were obtained, indicating the branches used for subtype identification as in a. c, d, Number of eggs laid by virgin females during a 1-hr (c) or 3-d (d) period in which either SAG or pC1 neurons were optogenetically silenced, shown as scatter plots with mean ± s.e.m. ***, P < 0.001, Wilcoxon test. e, Basal GCaMP6s signals in pC1 cell bodies in virgin and mated females, shown as scatter plots with mean ± s.e.m. ***, P < 0.001, Wilcoxon test.