Figure 1.
Comparison of the COVA1-16 Binding Mode with CR3022 and ACE2
(A) Crystal structure of the COVA1-16–RBD complex with the RBD in gray and the COVA1-16 Fab in cyan (heavy chain) and pink (light chain).
(B) The ACE2-binding site (PDB: 6M0J; left; Lan et al., 2020), the COVA1-16 epitope (this study, center), and the CR3022 epitope (PDB: 6W41; right; Yuan et al., 2020b) are highlighted in yellow.
(C) RBD residues in the COVA1-16 epitope. Epitope residues contacting the heavy chain are shown in orange and the light chain in yellow. Representative epitope residues are labeled. Residues that are also part of the CR3022 epitope are indicated by asterisks.
(D) The ACE2-RBD complex structure is aligned in the same orientation as the COVA1-16-RBD complex. The COVA1-16 light chain (pink) would clash with ACE2 (green) if they were to approach their respective RBD binding sites at the same time (indicated by a red circle).