Figure 4.
Sequence Conservation of the COVA1-16 Epitope and ACE2-Binding Site
(A and B) Sequence conservation of the RBD among 17 SARS-like CoVs (Figure S4) is highlighted on the RBD structure, with (A) COVA1-16 epitope and (B) ACE2-binding site indicated by the black outline. The backside of this view is shown in Figure S5A.
(C and D) Sequence conservation of the (C) COVA1-16 epitope and (D) ACE2-binding site is shown as a sequence logo.
(E) The binding kinetics of COVA1-16 IgG to RBDs from Guangdong pangolin CoV and bat CoV RaTG13 were measured by biolayer interferometry (BLI) with IgG on the biosensor and RBD in solution. The y axis represents the response. Dissociation constant (KD) values were obtained using a 1:1 binding model and are represented by the red lines. Representative results of two replicates for each experiment are shown.
See also Figures S4 and S5.