REAGENT or RESOURCE	SOURCE	IDENTIFIER
ExpiCHO Expression System Kit	Thermo Fisher Scientific	A29133
Expi293 Expression System Kit	Thermo Fisher Scientific	A14635
HyClone insect cell culture medium	GE Healthcare	SH30280.03
FreeStyle 293 expression medium	GIBCO	12338002
Opti-MEM I reduced serum media	GIBCO	51985091
Phosphate-buffered saline (PBS)	Thermo Fisher Scientific	14040133
Ni-NTA Superflow	QIAGEN	30450
DH10Bac competent cells	Thermo Fisher Scientific	10361012
CaptureSelect CH1-XL Affinity Matrix	Thermo Fisher Scientific	2943452010
Protein A column	Thermo Fisher Scientific	17040301
Chemicals and Recombinant Proteins
DpnI	New England Biolabs	R0176L
Trypsin	New England Biolabs	P8101S
Fugene 6 Transfection Regent	Promega	E2691
BirA Biotin-Protein Ligase Reaction Kit	Avidity	BIRA-500
Sodium chloride (NaCl)	Sigma-Aldrich	S9888
Tris Base	Sigma-Aldrich	11814273001
Concentrated hydrochloric acid (HCl)	Sigma-Aldrich	H1758
Sodium azide (NaN3)	Sigma-Aldrich	S2002
Bovine Serum Albumin (BSA)	Sigma-Aldrich	A9418
Tween 20	Fisher Scientific	BP337-500
PEImax	Polysciences	24765-1
Chemicals for protein crystallization	Hampton Research	N/A
Critical Commercial Assays
In-Fusion HD Cloning Kit	Takara	639647
KOD Hot Start DNA Polymerase	EMD Millipore	71086-3
PCR Clean-Up and Gel Extraction Kit	Clontech Laboratories	740609.250
QIAprep Spin Miniprep Kit	QIAGEN	27106
NucleoBond Xtra Maxi	Clontech Laboratories	740414.100
Deposited Data
X-ray coordinates and structure factors of COVA1-16 Fab	This study	PDB: 7JMX
X-ray coordinates and structure factors of COVA1-16 Fab in complex with SARS-CoV-2 RBD	This study	PDB: 7JMW
Cell Lines
ExpiCHO cells	Thermo Fisher Scientific	A29127
Expi293F cells	Thermo Fisher Scientific	A14527
HEK293F cells	Invitrogen	R79007
Sf9 cells	ATCC	CRL-1711
High Five cells	Thermo Fisher Scientific	B85502
Recombinant DNA
phCMV3-COVA1-16 IgG heavy chain	Brouwer et al., 2020	N/A
phCMV3-COVA1-16 Fab heavy chain	Brouwer et al., 2020	N/A
phCMV3-COVA1-16 light chain	Brouwer et al., 2020	N/A
pPPI4-SARS-CoV RBD	Brouwer et al., 2020	N/A
pPPI4-SARS-CoV-2 RBD	Brouwer et al., 2020	N/A
pPPI4-SARS-CoV	Brouwer et al., 2020	N/A
pPPI4-SARS-CoV-2	Brouwer et al., 2020	N/A
pFastBac-SARS-CoV-RBD	Yuan et al., 2020b	N/A
pFastBac-SARS-CoV-2-RBD	Yuan et al., 2020b	N/A
phCMV3-ACE2	This study	N/A
Software and Algorithms
HKL2000	Otwinowski and Minor, 1997	N/A
Phaser	McCoy et al., 2007	N/A
Coot	Emsley et al., 2010	N/A
Phenix	Adams et al., 2010	N/A
MolProbity	Chen et al., 2010	N/A
WebLogo	Crooks et al., 2004	N/A
PyMOL	Schrödinger, LLC	RRID:SCR_000305
Appion	Lander et al., 2009	N/A
DoG Picker	Voss et al., 2009	N/A
Relion	Zivanov et al., 2018	N/A
UCSF Chimera	Pettersen et al., 2004	N/A
Octet analysis software 9.0	Fortebio	https://www.moleculardevices.com/
Other
Fab-CH1 2nd generation (FAB2G) biosensors	ForteBio	Cat# 18-5019
Ni-NTA biosensors	ForteBio	Cat# 18-5102
Streptavidin (SA) biosensors	ForteBio	Cat# 18-5020
Negative stain EM grids, 400 mesh	Electron Microscopy Sciences	EMS400-CU