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. Author manuscript; available in PMC: 2020 Nov 25.
Published in final edited form as: Nature. 2020 Apr 1;580(7803):381–385. doi: 10.1038/s41586-020-2156-5

Figure 3: LRP1 mediates tau uptake in neurons.

Figure 3:

a, Representative images of WT or LRP1 sgRNA iPSN after tau uptake (green, Tau-488; red, neuronal marker Tuj-1; blue, DAPI; scale bar 20μm). b, Uptake of 2N4R monomers and oligomers in CRISPRi iPSN (n=10). c, Uptake of 2N4R tau (WT vs. mtRAP; p-value = >0.9999) or Tfn (ANOVA; p-value = 0.7815) in the presence of RAP or mtRAP (n=7). All experiments in a-c were performed over three independent experiments and normalized to WT uptake (100%). Data expressed as mean ± s.d. One-way ANOVA with Tukey’s method, two-sided was performed to determine significance. Displayed is the multiple comparison against WT, ns=not-significant, ****=p-value < 0.0001. Comparison to NT sgRNA resulted in the same level of statistical significance.