Table 3.
Protein yields of various RBD constructs using the MagBead process. Numbers represent yields from independent experiments. RBDs represents the same RBD amino acid sequence as the Sinai construct, while RBDr represents the same RBD amino acid sequence as the Ragon construct. n/a: not applicable as only one purification was performed.
Condition | Protein Yield (mg/l) | Mean (mg/l) |
---|---|---|
X22 (Sinai) | 62.1, 72.5 | 67.3 ± 7.4 |
M67 (RBDs-His6) | 79.8 | n/a |
X24 (Ragon) | 15.6, 19.2, 21.8 | 18.9 ± 3.1 |
M68 (RBDr-3C-His8-SBP) | 78.3 | n/a |
M69 (RBDr-3C-His8) | 58.8 | n/a |