Figure 3.

T Cell Immune Responses in Nanoparticles Vaccinated BALB/c Mice

(A–C) BALB/c mice were euthanized at six weeks post boost vaccination. Splenocytes were incubated with SARS-CoV-2 S peptides pool. The percentages of IFN-γ⁺, IL-2⁺ and TNF-α⁺ CD8⁺ T cells were determined by ICCS.

(D and E) Splenocytes were stimulated with S peptide pool as in (A). The percentages of IFN-γ⁺ and IL-4⁺ CD4⁺ T cells were determined by ICCS.

(F and G) Splenocytes were stimulated with S peptide pool. ELISpot assays were conducted for IFN-γ and IL-4 secretion in splenocytes. Experiments were conducted independently in triplicates. Data represented as mean ± SEM (n = 4). Adjusted p values were calculated by one-way ANOVA with Tukey’s multiple comparisons test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

See also Figure S3.