REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP	Invitrogen	Cat#31430; RRID: AB_228307
Goat anti-Monkey IgG (H+L) Secondary Antibody, HRP	Invitrogen	Cat#PA1-84631; RRID: AB_933605
Rabbit Polyclonal anti-SARS-CoV-2 Nucleoprotein (N) Antibody	Sino Biological	Cat#40143-T62
Goat Anti-Rabbit IgG Secondary Antibody (HRP)	Sino Biological	Cat#SSA004
CD3e Monoclonal Antibody (145-2C11), FITC	eBioscience	Cat#11-0031-82; RRID: AB_464882
Alexa Fluor 700 anti-mouse CD4 Antibody	Biolegend	Cat#100429; RRID: AB_493698
Brilliant Violet 510 anti-mouse CD8a Antibody	Biolegend	Cat#100752; RRID: AB_2563057
FITC anti-mouse/human CD44 Antibody	Biolegend	Cat#103006; RRID: AB_312957
PE anti-mouse CD62L Antibody	Biolegend	Cat#104407; RRID: AB_313094
PE-Cy7 Hamster Anti-mouse CD69 Antibody	BD Biosciences	Cat#552879; RRID: AB_394508
Ultra-LEAF Purified anti-mouse CD28 Antibody	Biolegend	Cat#102116; RRID: AB_11147170
APC anti-mouse IFN-γ Antibody	Biolegend	Cat#505810; RRID: AB_315404
PE anti-mouse IL-4 Antibody	Biolegend	Cat#504103; RRID: AB_315317
APC anti-mouse/human CD45R/B220 Antibody	Biolegend	Cat#103212; RRID: AB_312997
PerCP/Cyanine5.5 anti-mouse CD11c Antibody	Biolegend	Cat#117327; RRID: AB_2129642
CD209b (SIGN-R1) Monoclonal Antibody (eBio22D1 (22D1)), APC	eBioscience	Cat#17-2093-80; RRID: AB_11149350
CD11b Monoclonal Antibody (M1/70), PE-Cyanine7	eBioscience	Cat#25-0112-82; RRID: AB_469588
Brilliant Violet 421 anti-mouse CD169 (Siglec-1) Antibody	Biolegend	Cat#142421; RRID: AB_2734202
F4/80 Monoclonal Antibody (BM8), APC	Invitrogen	Cat#MF48005; RRID: AB_10375306
FITC anti-mouse IgG1 Antibody	Biolegend	Cat#406606; RRID: AB_493293
PE anti-mouse IgG2b Antibody	Biolegend	Cat#406707; RRID: AB_2563380
FITC anti-mouse CD38 Antibody	Biolegend	Cat#102705; RRID: AB_312926
Bacterial and Virus Strains
E.coli BL21	Takara	Cat#9126
SARS-CoV-2	This paper	hCoV-19/CHN/SYSU-IHV/2020 strain; GISAID: EPI_ISL_444969
ZIKV	Dr. Bishi Fu	Strain MR766
Biological Samples
Serum samples from BALB/c mice	This paper	N/A
Serum samples from hACE2 mice	This paper	N/A
Serum samples from rhesus macaques	This paper	N/A
Lung samples from hACE2 mice	This paper	N/A
Blood samples from healthy individuals	Guangzhou Blood Center, Guangzhou	http://www.gzbc.org/
Chemicals, Peptides, and Recombinant Proteins
4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI)	ThermoFisher	Cat#D1306
eBioscience Fixable Viability Dye eFluor 780	Invitrogen	Cat#65-0865
Isopropyl β-D-1 thiogalactopyranoside (IPTG)	Takara	Cat#9030
Ni Sepharose excel	GE Healthcare	17-3712-01
Sigma adjuvant System (SAS)	Sigma-Aldrich	Cat#S6322
eBioscience Intracellular Fixation & Permeabilization Buffer Set	Invitrogen	Cat#88-8824
Brefeldin A	Topscience	T6062; CAS: 20350-15-6
Monensin sodium salt	Topscience	T1033; CAS: 22373-78-0
Collagenase from Clostridium histolyticum-Type IV	Sigma-Aldrich	V900893; CAS9001-12-1
ELISA Stop Solution	Solarbio	C1058
eBioscience TMB Solution	eBioscience	Cat#00-4201
Carboxymethylcellulose sodium salt (CMC)	Sigma-Aldrich	21902; CAS9004-32-4
TrueBlue HRP Substrate	KPL	50-78-02
Paraformaldehyde	Sigma-Aldrich	P6148; CAS30525-89-4
Penicillin-Streptomycin, Liquid	ThermoFisher	Cat#15140122
Fetal Bovine Serum (FBS)	ThermoFisher	Cat#10270-106
Biotin-Protein Ligase / BirA Enzyme	GeneCopoeia	BI001
Brilliant Violet 421 Streptavidin	BioLegend	Cat#405225
Recombinant SC-Ferritin protein	This paper	N/A
Recombinant ST-RBD protein	This paper	N/A
Recombinant ST-HR protein	This paper	N/A
SARS-CoV-2 Spike Glycoprotein Peptides Pool	GenScript	Cat#RP30020
HCoV-OC43 Spike glycoprotein Peptides Pool	GenScript	Cat#RP30011
HCoV-229E Spike Glycoprotein Peptides Pool	GenScript	Cat#RP30010
Recombinant Human GM-CSF	PeproTech	Cat#300-03
Recombinant Human IL-4	PeproTech	Cat#200-04
Recombinant Human TNF-α	PeproTech	Cat#300-01A
Recombinant Human IL-2	PeproTech	Cat#200-02
Recombinant Human IL-10	PeproTech	Cat#200-10
Critical Commercial Assays
RNeasy Mini Kit	QIAGEN	Cat#74104
SARS-CoV-2 RNA detection kit (PCR-Fluorescence Probing)	Da An Gene Co.	DA0931
Monkey IFN-γ ELISPOT kit	MabTech	3421M-4AST-2
Monkey IL-4 T cell ELISPOT kit	U-Cytech	Cat#CT128-PR2
Mouse IFN-γ ELISPOT kit	Dakewe	Cat#2210005
Mouse IL-4 precoated ELISPOT kit	Dakewe	Cat#2210402
Human IFN-γ precoated ELISPOT kit	Dakewe	Cat#2110005
Pierce Rapid Gold BCA Protein Assay Kit	ThermoFisher	Cat#A53225
Luciferase Assay System	Promega	Cat#E4550
Experimental Models: Cell Lines
HEK293T	ATCC	CRL-3216; RRID: CVCL_0063
HeLa	ATCC	CCL-2; RRID: CVCL_0030
Vero E6	ATCC	CRL-1586; RRID:CVCL_0574
FreeStyle CHO-S cells	Invitrogen	Cat#R800-07
Experimental Models: Organisms/Strains
Transgenic hACE2 mice (C57BL/6)	GemPharmatech Co, Ltd	N/A
C57BL/6 mice	Guangdong Medical Laboratory Animal Center	N/A
BALB/c mice	Guangdong Medical Laboratory Animal Center	N/A
Rhesus macaques	Guangdong Landau Biotechnology Co, Ltd	N/A
IFNαR−/− mice (C57BL/6)	Jackson Laboratory	N/A
Oligonucleotides
SARS-CoV-2 nucleocapsid (N) qPCR Forward Primer:5′- CAGTAGGGGAACTTCTCCTGCT-3′	Da An Gene Co.	DA0931
SARS-CoV-2 nucleocapsid (N) qPCR Reverse Primer:5′-CTTTGCTGCTGCTTGACAGA-3′	Da An Gene Co.	DA0931
SARS-CoV-2 nucleocapsid (N) Probe: 5′-FAM-CTGGCAATGGCGGTGATGCTGC-BHQ1-3′	Da An Gene Co.	DA0931
Recombinant DNA
pET28a-SC-Ferritin-6His	This paper	N/A
pcDNA3.1-SP-ST-RBD-6His	This paper	N/A
pcDNA3.1-SP-ST-HR-6His	This paper	N/A
psPAX2	Dr. Didier Trono	Addgene Plasmid #12260
pHIV-Luciferase	Dr. Bryan Welm	Addgene Plasmid #21375
SARS-CoV Spike Gene	Dr. Wenlin Huang	GenBank: AAP13567.1
MERS-CoV Spike Gene	Dr. Shibo Jiang	GenBank: AFS88936.1
HCoV-229E Spike Gene ORF cDNA clone expression plasmid	Sino Biological	Cat# VG40605-CF; GenBank: APT69883.1
HCoV-OC43 Spike Gene ORF cDNA clone expression plasmid	Sino Biological	Cat# VG40607-CF; GenBank: AVR40344.1
RATG13 Spike Gene	This paper	GenBank: QHR63300.2
Mouse CD64/FCGR1 ORF mammalian expression plasmid	Sino Biological	Cat#MG50086-ACR
Mouse CD32/Fc gamma RII/FCGR2 Gene ORF cDNA clone expression plasmid	Sino Biological	Cat#MG50030-ACG
Rhesus CD64/FCGR1 ORF mammalian expression plasmid	Sino Biological	Cat#CG90017-ACR
Cynomolgus CD32b/FCGR2B/Fc gamma RIIB Gene ORF cDNA clone expression plasmid	Sino Biological	Cat#CG90014-ACG
Software and Algorithms
GraphPad Prism v8.0 software	GraphPad	https://www.graphpad.com/scientific-software/prism/
BD LSRFortessa cell analyzer	BD Biosciences	http://www.bdbiosciences.com/in/instruments/lsr/index.jsp
FlowJo v10	Tree Star	https://www.flowjo.com/
Image Studio Lite v4.0	LI-COR Biosciences	https://www.licor.com/bio/image-studio-lite/
QuantStudio 7 Flex System	ThermoFisher	https://www.thermofisher.com/order/catalog/product/4485701#/4485701
GloMax 96 Microplate Luminometer Software v1.9.3	Promega	https://www.promega.com/resources/software-firmware/detection-instruments-software/promega-branded-instruments/glomax-96-microplate-luminometer/
SkanIt SW for Microplate Readers	ThermoFisher	https://www.thermofisher.com/order/catalog/product/5187139?SID=srch-srp-5187139
ImmunoSpot software v5.1.34	Cellular Technology Ltd	http://www.immunospot.com/ImmunoSpot-analyzers
Other
Superose 6 Increase 10/300 GL	GE Healthcare	29091596
Amicon Ultra-15 Centrifugal Filter Unit	Millipore	UFC900396
Talos L120C microscope	ThermoFisher	https://www.thermofisher.com/cn/zh/home/electron-microscopy/products/transmission-electron-microscopes/talos-l120c-tem-ls.html
Leica RM2235	Leica	https://www.leicabiosystems.com/cn/histology-equipment/microtomes/products/leica-rm2235/
Olympus BX63	Olympus	https://www.olympus-lifescience.com.cn/zh/microscopes/upright/fluorescence/