Fig. 1.

HMGB1 mobilization from hepatocytes of human and mice during HFD feeding. a Representative confocal immunofluorescence imaging of liver tissue of WT mice after 2, 8 and 16 weeks of LFD or HFD feeding. HMGB1 (red), actin (green) and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue). Scale bars represent 20 μm. × 60 magnification. b Serum HMGB1 levels in WT mice after LFD or HFD feeding for 2, 8 and 16 weeks. N = 8/gp; data represent mean ± SEM; *P < 0.05 between indicated groups. c Representative Western blot images of HMGB1 in whole cell lysates of human (left) and WT mouse (right) hepatocytes at 24 h after up to 800 μM palmitic acid (PA) treatment. Images representative of at least three repeats. Ctrl = no treatment. GAPDH was used a loading control. d HMGB1 levels in the culture medium of human hepatocytes with PA exposure. Data presented as mean ± SEM. Data representative of at least three repeats. *P < 0.05 between indicated groups