Fig. 5.

Deletion of HC-HMGB1 down-regulates hepatic gene expression related to β-oxidation in WT and HC-HMGB1^−/− (KO) mice up to 16 weeks and mouse hepatocytes at 24 h after PA treatment. a Relative gene expression of hepatic CPT-1, MCAD, LCAD and VLCAD in WT and HC-HMGB1-KO mice on LFD or HFD (n = 4 for each group). b Relative gene expression of CPT-1α, MCAD, LCAD and VLCAD in mouse hepatocytes at 24 h after PA treatment (800 µM). c OCR in WT and HC-HMGB1^−/− mouse hepatocytes. OCR tracing includes vertical lines indicating time of addition of mitochondrial inhibitors: oligomycin (4 µM), FCCP (1 µM), and rotenone (1 µM). OCR was normalized to protein content and is shown as fold changes of normoxic control. Data are mean ± SEM, graphs show collated data from 10 individual measurements from at least three separate experiments. *P < 0.05 between indicated groups; NS not significant