Fig. 3.

Extracellular vesicular Wnt7b can be transferred to HUVECs. a The internalization of CC-derived EVs by HUVECs was evaluated using a fluorescence confocal microscope. PKH67 (green fluorescent dye)-labeled EVs derived from HPV 16 E6-KD cells (SiHa or CaSki cells), HPV 18 E6-KD cells (HeLa or SW756 cells) or their corresponding control cells (EV/NC) were all effectively taken up by HUVECs. Untreated HUVECs were used as the negative control. b qRT-PCR analysis showed that Wnt7b mRNA levels were significantly lower in HUVECs treated with EV/E6-KD than in those treated with EV/NC derived from the four HPV 16/18-positive cell lines. c Western blot analysis showed very low Wnt7b protein expression in untreated HUVECs. Wnt7b protein levels were increased when treated with EV/NC derived from the four HPV 16/18-positive CC cell lines, while the treatment of EV/E6-KD inhibited such promotion of Wnt7b protein expression. * P < 0.05, ** P < 0.01