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. 2020 Nov 25;39:259. doi: 10.1186/s13046-020-01769-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — DNMT3a activates JAK2/STAT3 signaling pathway by suppressing SOCS3 expression. a Western blot results of genes involved in JAK/STAT3 signaling pathway after deletion of DNMT3a. b PGL3-SOCS3 promoter was co-transfected with different doses of MYC-DNMT3a, the activity of SOCS3 promoter was measured by luciferase. Renilla was used as control. c Diagram of CpG island in SOCS3 promoter. d ChIP-qPCR results of DNMT3a on SOCS3 promoter and its nearby non-CpG region. IgG was used as control. e Detection of SOCS3 promoter methylation with Methylated-DNA IP Kit. f Detection of SOCS3 promoter methylation after depleting DNMT3a. *P < 0.05, **P < 0.01, ***P < 0.001