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. 2020 Nov 25;13:595. doi: 10.1186/s13071-020-04484-6

Table 3.

Comparison of full-length Blastocystis SSU rRNA gene sequences generated in this study by MinION sequencing to Illumina MiSeq sequences from the same sample and closest full-length match available on GenBank

Specimen ID Blastocystis subtype Length of sequence generated by MinION in this study (GenBank accession number) Similarity to Illumina MiSeq sequencea (%) Similarity to closest match available in GenBank (sequence length, accession number)
1 ST1 1766 bp (MT898451) 100 99.8 (1770 bp, U51151)
2 ST4 1772 bp (MT898452) 100 100 (1730 bp, AY590114)
3 ST4 1773 bp (MT898453) 100 99.9 (1730 bp, AY590114)
4 ST11 1762 bp (MT898454) 100 99.9 (989 bp, GU256903)
1763 bp (MT898455) 100 98.6 (989 bp, GU256929)
5 ST10 1770 bp (MT898456) 99.8 99.5 (1728 bp, KC148207)
6 ST10 1770 bp (MT898457) 99.8 99.5 (1728 bp, KC148207)
ST14 1771 bp (MT898458) 99.8 99.6 (1772 bp, KC148205)
7 ST14 1771 bp (MT898459) 99.8 99.7 (1772 bp, KC148205)

aIllumina MiSeq sequence corresponds to the approximately 480 bp region of the SSU rRNA gene amplified using the primers reported in [11] using methods reported in [12]