Figure 3.

Fibroblast migratory response to a FGF gradient. a) Schematic of fibroblast chemotaxis due to FGF gradients during wound healing. b) Migration velocity relative to the negative control for FGF loading concentrations (c_load) of 5, 10, 15, 25, 50, and 100 ng mL⁻¹ showing an optimal concentration at 50 ng mL⁻¹. c) FEA results showing the temporal evolution of the FGF concentration in the cell-seeding chamber (location indicated by the red dot) for FGF loading concentrations of 10, 25, 50, and 100 ng mL⁻¹ (n = 3, Student’s t-test, p-value notation: * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001).