Fig. 1.

Overview of spermatogenesis in the Drosophila testis. (A) Cartoon depicting spermatid development in Drosophila. Germline stem cells (red) are located at the hub (green) of the testis. (A′) Spermatogonia divide mitotically four times to form a 16-cell cyst. (A″) These cysts undergo a growth phase of ∼3 days before undergoing two rounds of meiosis to form 64-cell cysts. Following this, each cell elongates a tail to form bundles of mature spermatids. (B-B″) Immunofluorescence shows that ring canals marked by Pav::GFP (B′) and the fusome stained with Adducin antibody (B″) are present throughout spermatogenesis. Scale bar: 100 μm; inset 10 μm.