Fig. 3.

RCs allow for sharing of some, but not all, proteins. (A-L) Fluorescence loss in photobleaching (FLIP) demonstrated that not all GFP-tagged proteins move between the cells in a 16-cell cyst. Several cells within a 16-cell cyst (red outline) expressing GFP or a GFP-tagged protein were continuously bleached (yellow outline) over the course of 1 h. Protein movement was determined by a loss in GFP fluorescence from neighboring cells within that cyst (white outline), indicating that GFP from non-bleached cells moved into the bleached region. (M-P) Quantification of GFP from the representative images (A-L) in the bleached (solid line), non-bleached (dotted line) and neighboring (dashed line) regions in a spermatocyte cyst. FLIP was detected for GFP, GFP::Oda, GFP::Men-B but not GFP::CaM. Mean fluorescence intensity (AU) is plotted with respect to time. Intermittent peaks on the graphs represent quick recovery of GFP in the sample while the microscope switches between capture and bleach modes. Scale bar: 10 μm.