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. 2020 Nov 17;147(22):dev195974. doi: 10.1242/dev.195974

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© 2020. Published by The Company of Biologists Ltd

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Fig. 3. — ASP expression of novel Hh target genes. (A-D) Compared with control (A), fluorescence of ptc>nucGFP (green) is decreased by expression of Ptc (B, btl-Gal4>Ptc), smo RNAi (C, btl-Gal4>smoRNAi), and increased by expression of Hh:CD2 (D, btl-Gal4 >Hh:CD2). ASP is outlined by white dashed lines. (E-H) Compared with control (E), α-En antibody staining is decreased by expression of Ptc (F, btl-Gal4>Ptc), smo RNAi (G, btl-Gal4>smoRNAi), and increased by expression of Hh:CD2 (H, btl-Gal4 >Hh:CD2). (I-L) Compared with control (I), α-Ser antibody staining is decreased in the ASP by Ptc expression (J, btl-Gal4>Ptc) and smo RNAi (K, btl-Gal4>smoRNAi), and is increased by expression of Hh:CD2 (L, btl-Gal4>Hh:CD2). (M-P) Compared with control (M), α-Ct antibody staining is increased in the ASP by Ptc expression (N, btl-Gal4>Ptc) and smo RNAi (O, btl-Gal4>SmoRNAi) and is decreased by expression of Hh:CD2 (P, btl-Gal4>Hh:CD2). (Q-T) Compared with control (Q), α-Aop antibody staining is increased in the ASP by Ptc expression (R, btl-Gal4>Ptc) and smo RNAi (S, btl-Gal4>smoRNAi) and is decreased by expression of Hh:CD2 (T, btl-Gal4>Hh:CD2). Scale bar: 20 μm.