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. 2020 Nov 17;147(22):dev195974. doi: 10.1242/dev.195974

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Fig. 7. — Cytonemes required for Hh transport and signaling in ASP. (A-E) Hh:GFP from disc distributes at similar levels in control (A, btl>+; Gal80^ts) and disp knockdown (B, btl>dispRNAi; Gal80^ts) ASPs, but at reduced amounts in SCAR (C, btl>ScarRNAi; Gal80^ts) and dia (D, btl>diaRNAi; Gal80^ts) knockdown ASPs. E shows Quantification of Hh:GFP puncta in control and knockdown ASPs. n=5-7. (F-J) α-Ptc staining (green) in control (F, btl>+; Gal80^ts), dia (G, btl>diaRNAi; Gal80^ts), nrg (H, btl>nrgRNAi; Gal80^ts) and syt4 (I, btl>syt4RNAi; Gal80^ts) ASPs. n=12. *P<0.05, **P<0.005 (unpaired two-tailed Student's t-test). n.s. not significant. Scale bars: 25 μm.