Extended Data Fig. 3. NachBac expression in Arc^AgRP neurons increases neuron activity levels and promotes massive obesity.

a, Expression GFP (green column) and c-Fos (red column) and their colocalization (merged column) with magnified pictures showing the boxed area in the merged pictures from AgRP-Cre mice injected with Cre-dependent GFP (top two rows) or NachBac (bottom two rows) when treated fed ad libitum chow (the first and third rows) or fasted (the second and forth row). It is notable that the vast majority of NachBac-expressing neurons exhibited c-Fos regardless of fed or fasted, n=3 as presented in Fig. 2c. b, Comparison in lean mass between control and mice with NachBac expression in AgRP^NaChBac mice 11 weeks after viral delivery (n=7 for control and 8 for NachBac, t=0.1576, df=13, p=0.8772, 2-sided unpaired Student’s t test). c, Comparison in body weight when mice were placed in CLAMS chambers for O2 consumption measurements during the 1^st week after viral delivery (n=7 for control and 8 for NachBac, t=0.5734, df=9, p=0.5804, 2-sided unpaired Student’s t test). d-f, Real time measurements of food intake (d), locomotion (e) and O2 consumption (f) over 3 days. 3V: the third ventricle. Data presented as mean+/−sem.