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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Nat Metab. 2020 Jul 27;2(8):763–774. doi: 10.1038/s42255-020-0229-2

Extended Data Fig. 4. AgRP neuron ablation causes no difference in obesity development by NachBac.

Extended Data Fig. 4

a, Representative pictures showing immunostraining for AgRP in PVH (top panels) and Arc (bottom panels) in adult AgRPDTR/+ mice treated with either saline (left panels) or DTX (right panels) at day 3, repeated in 3 mice. b, NPY-GFP transgene was bred into AgRPDTR/+ mice, and DTX treatment led to almost complete ablation of NPY neurons in the Arc (arrows), repeated in one additional mouse. c-d, Expression of NachBac in the Arc of Vgat-Cre mice with AgRP neuron lesion caused reduced locomotion shown in representative traces (c) and comparison in locomotion between groups (d, n=5 each/groups, t=9.366, df=8, **p<0.0001, 2-sided Student’s t test). e, i.c.v. leptin infusion was confirmed by induction of p-STAT3 shown by immunostaining (bottom panels), compared to control PBS infusion (top panels), repeated in 3 mice. 3V: the third ventricle. Scale bar in b: 400 μM and arrows in panel b pointing to residual NPY neurons. Data presented as mean+/−sem.