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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Nat Metab. 2020 Jul 27;2(8):763–774. doi: 10.1038/s42255-020-0229-2

Extended Data Fig. 9. Kir2.1 expression in Arc GABA+ neurons reduces neuron activity and normalizes body weight of ob/ob mice.

Extended Data Fig. 9

a, Vgat-Cre::ob/ob mice received either control vector (top panels) or AAV-DIO-Kir2.1-p2A-dTomato (bottom panels) to the Arc, and immunostraining was performed 4 weeks after viral delivery for c-Fos (green column) in response to overnight fasting (the magnified pictures on the right showing details of the boxed areas in the merged pictures as indicated). b, Comparison in the number of Arc neurons with c-Fos expression between groups (n=3 each/group, t=8.990, df=4, **p=0.0008, 2-sided unpaired Student’s t test). c-e, CLAMS measurements of feeding (c), O2 consumption (d) and locomotion (e) in mice with control or Kir2.1 vector delivery to bilateral Arc of Vgat-Cre::ob/ob mice. The CLAMS measurement was performed during the first week after viral delivery when body weight difference between groups was minimal, n=5 each/group as presented in Fig. 7c7h. f, AgRP-Cre::ob/ob mice received either control vector (top panels) or kir2.1 vectors (bottom panels) to the Arc, and immunostraining was performed 4 weeks after viral delivery for c-Fos (green column) in response to overnight fasting (the magnified pictures on the right showing details of the boxed areas in the merged pictures as indicated), n=3 as presented in Fig. 7j and panel g. g-i, Comparison in the number of Arc neurons expressing c-Fos between the groups (g, n=3 each/group, t=10.15, df=4, **p=0.0005, 2-sided unpaired Student’s t test), fat mass (h, n=7 (control) or 8 (Kir2.1), t=0.5465, df=13, p=0.5940, 2-sided unpaired Student’s t test) and lean mass (i, n=7 (control) or 8 (Kir2.1), t=0.3036, df=14, p(two-tailed)=0.7659, 2-sided unpaired Student’s t test) at the 11th week after viral delivery. 3V: the third ventricle. Data presented as mean+/−sem.