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. 2020 Nov 24;11:5964. doi: 10.1038/s41467-020-19617-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b The binding affinity of EBV gHgL to LBD (a) and KSHV gHgL to LBD (b), as measured using SPR. EphA2 LBD was immobilized to chip CM5 and the binding affinities of various concentrations of EBV gHgL or KSHV gHgL were tested. The kinetic profiles are shown. KD values shown are the mean ± SEM of three independent experiments. The binding profiles were plotted by GraphPad Prism 8.0. c Analytical gel-filtration analyses of EBV gHgL–LBD (red), EBV gHgL (blue), and LBD (cyan) as measured using calibrated Superdex^® 200 Increase 10/300 GL columns (GE Healthcare). The chromatographs and SDS-PAGE profiles for each pooled sample (peaks 1–4) are shown. The SDS-PAGE results showed peak 1 contains only EBV gHgL proteins, indicating EBV gHgL and LBD proteins did not form a complex in the gel-filtration assays. d Analytical gel-filtration analyses of KSHV gHgL–LBD (red), KSHV gHgL (blue), and LBD (cyan) as measured using calibrated Superdex^® 200 Increase 10/300 GL columns (GE Healthcare). The SDS-PAGE results showed peak 1 contains both KSHV gHgL and LBD proteins, indicating these two proteins could form a complex in the gel-filtration assays. The gel-filtration chromatographs were plotted by OriginPro 8.5. Experiments have been repeated twice and similar results were observed. Source data are provided as a Source Data file.