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. 2020 Nov 24;10:407. doi: 10.1038/s41398-020-01087-8

Fig. 3. Generation of the brain-specific NTRK1 E495K knock-in (cKI) mice.

Fig. 3

a Gene targeting strategy. b Southern blot of the targeted ES cells. c PCR genotyping of the Ntrk1 recombined alleles. d Genomic DNA sequencing of the wild-type and KI alleles. e Validation of the anti-NTRK1 antibody specificity by western blots. Whole brain tissues were used for knockouts embryos (embryonic day 18). Cerebral cortices were used for the adult mouse samplesf) Expression levels of the NTRK1 protein among genotypes. C control. g Body weight. N = 13 for each genotype. h Hot plate test to assess the pain sensitivity. N = 13 for each genotype. Error bars, mean ± s.e.m.