(A) Ion trajectory simulations of 1000 ions of m/z 1034 traversing the ion escalator using 16/s TW speed and 40 Vpp TW amplitude. (B) Simulated mobiligrams of negative Agilent tune mix ions (m/z 602 (red), 1034 (green), 1334 (blue), 1634 (orange), 1934 (pink), 2234 (purple), 2534 (light gray), and 2834 (dark gray)) using 16 m/s TW speed. Each simulation in panel B utilized 1000 ions per m/z. Data sets were normalized to the m/z with the highest intensity. Guard = −5 V, RF = 300 Vpp, P = 3.5 Torr helium.