(A) Separation of Agilent tune mix negative ions using one, two, three, and four ion levels. (B) Ion transmission efficiencies across successive levels normalized to the intensity obtained using one level. TWsep speed = 192 m/s, TWsep amplitude = 27 Vpp, TWesc speed = 24 m/s, TWesc amplitude = 22 Vpp, guard = −35 V, P = 2.8 Torr helium.