Fig. 9.
Images with increased number of time windows. a) Top: PSF obtained with the Lissajous scanning while driving the TAG lens at a frequency of only 142 kHz, with an axial scan range of 9 µm, and a 60x objective lens. Bottom: PSF with the TAG lens off (conventional confocal with a piezo stage). b) Images of a flower pollen, acquired with the same imaging conditions, at 6 different axial positions are shown with color coding for depth information. Note that the acquired 3D stacks were reconstructed using 36 scans. Scale bar 5 µm, Excitation wavelength at 488 nm, voxel size 0.078 × 0.078 × 0.32 µm3, fTAG = 142705 Hz, Lissajous period 2π.