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. 2020 Nov 24;11:493. doi: 10.1186/s13287-020-02019-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Two-step serum/feeder-free hematopoietic differentiation from human embryonic stem cells (ESCs) displayed high colony-forming unit (CFU) potential and generated a significant portion of hematopoietic stem cell (HSC)-like cells. 7-day embryoid body (EB) maturation followed by 8-day organoid differentiation provided a the lowest percentage of primitive (CD34− CD235a+) protocol and b the highest percentage of definitive (CD34+ CD235a−) progenitors. c Matrigel or gelatin coating did not result in a statistically significant difference in hematopoietic cell emergence. d CFU potential, e HSC-like, and f hemogenic endothelium cell (HE) fraction in different experimental groups (n = 3, *p < 0.05, **p < 0.01)